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Subcellular Protein Fractionation Kit
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发布时间：2025-01-10
原装现货|Subcellular Protein Fractionation Kit/亚细胞蛋白分离试剂盒
| 产品编号：78840 查看说明书产品名称：原装现货|Subcellular Protein Fractionation Kit/亚细胞蛋白分离试剂盒.0订购此产品供应商：Pierce规格：kit目录价：5402库存状态：现货CAS编号：应用范围：种属来源：相关信息：Subcellular Protein Fractionation KitSegregate and enrich proteins from five cellular compartments.The Thermo Scientific Subcellular Protein Fractionation Kit enables segregation and enrichment of proteins from five different cellular compartments.The kit enables the stepwise separation and extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from mammalian cultured cells. Extracts obtained with the Subcellular Protein Fractionation Kit are compatible with a variety of downstream applications including Western blotting, protein assays, electrophoretic mobility shift assays and reporter-gene and enzyme-activity assays.Fractionation of subcellular proteins enables protein localization assessment and protein enrichment from specific cellular compartments. The Thermo Scientific Subcellular Protein Fractionation Kit includes a combination of reagents for stepwise lysis of cells into functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hours. Extracts from each subcellular compartment generally have less than 15% contamination between fractions, which is sufficient purity for most experiments studying protein localization and redistribution.Highlights of the Subcellular Fractionation Kits:Concise – extract functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hoursConvenient – perform a simple procedure without using ultracentrifugation over gradientsComplete – obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kitCompatible – use extracts for downstream applications such as protein assays, Western blotting, gel-shift assays and enzyme activity assaysApplications for subcellular fractionation:Extract and enrich proteins from different cellular compartmentsIsolate cytoplasmic, nuclear, membrane and cytoskeletal proteins from the same cellsProfile cells for proteins from the cytoplasm, nucleus, membrane and cytoskeletonStudy protein translocationProduct Details:The Subcellular Protein Fractionation Kit contains four extraction buffers, a stabilized nuclease and Thermo Scientific Halt Protease Inhibitor Cocktail. Each kit has enough reagents to fractionate 50 cell pellets of 20µL each, equivalent to approximately 2g of cell paste. The first reagent added to a cell pellet causes selective membrane permeabilization, releasing soluble cytoplasmic contents. The second reagent dissolves plasma, mitochondria and ER-golgi membranes but does not solubilize the nuclear membranes. After recovering intact nuclei by centrifugation, a third reagent yields the soluble nuclear extract. An additional nuclear extraction with micrococcal nuclease is performed to release chromatin-bound nuclear proteins. The recovered insoluble pellet is then extracted with the final reagent to isolate cytoskeletal proteins.Subcellular Protein Fractionation KitFormulation: Multi-component kitSufficient For: 50 samples each containing 2 million cells (20µL packed)Kit Contents:Cytoplasmic Extraction Buffer, 10mLMembrane Extraction Buffer, 10mLNuclear Extraction Buffer, 10mLPellet Extraction Buffer, 5mLMicrococcal Nuclease, 150µLCalcium Chloride, 100mM, 250µLHalt Protease Inhibitor Cocktail (100X), 350µL保存条件：按原厂要求保存 |
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发布于 ： 2025-01-10
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